FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13584587
Sequences flagged as poor quality0
Sequence length35-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3350932.466714667144463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6050.014.7229311
ATCGGAA7150.013.0704342
TATGCCG8450.09.9864345
TCGGAAG9700.09.1738453
CGGAAGA13550.07.223684
CGTATGC10555.820766E-116.291241643
TCGTATG11204.4201443E-105.80975642
CTCGTAT10301.4897523E-85.639528841
TCTCGTA10507.5738717E-75.101359440
CGTTGGA29150.05.0419091
Produced by FastQC (version 0.11.5)