FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25921803
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA53300.09.83523751
AACGGGA50650.09.1354738
ACGGGAT36650.09.1046749
AAACGGG62650.08.7706427
GAAACGG63550.08.6114266
GGAAACG64750.07.8004495
CGGGATT35350.07.74034510
GCCACCG63350.06.91847528
ACCGCGC22400.06.853082731
CACCGCG24800.06.728131330
GCGTGAG67250.06.68238322
CGTGAGC69900.06.651885523
CCCAAAG272350.06.53611561
ACAGGCG94900.06.51668318
CGCGCCC17650.06.302643333
AACGGGT15550.06.2946198
CGGGATA10255.820766E-116.293884310
CCACCGC49400.06.214952529
CCCTAAA77900.06.18529651
CGCCCGG33250.06.156214735
Produced by FastQC (version 0.11.5)