FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842957
Sequences flagged as poor quality0
Sequence length25-290
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCCG18652.6707488E-45320.7686255-259
ATACAAT124251.5199158E-45191.229260-264
CGATTGC16453.622781E-44612.982255-259
TCGATTG22904.0265755E-44333.29255-259
TTCCTGG169602.8319037E-43803.1262260-264
GCGCTTG15906.2960276E-43527.5374245-249
TACAATG96405.488944E-43345.4888260-264
ATTCCGA17659.965624E-42811.1143250-254
ACAATGG102000.00102413782529.4517260-264
AGGGTAC37500.00125959142457.182250-254
CAATGGC87950.00114211742444.6094260-264
CCCCCCC24450.02398.2532245-249
TACGCAG10300.00155061942277.1763240-244
CATTCCG42500.00161782512168.1016250-254
TCCTGGC152850.00137987672109.945260-264
GGGTACC24650.00194361952012.8263250-254
ATTGCCT98600.00200950591869.053255-259
TTCGATT101700.00213783331812.081255-259
GATATAG58250.0030388071581.8767250-254
ACTTAGG60600.00328889651520.5332250-254
Produced by FastQC (version 0.11.5)