FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12385471
Sequences flagged as poor quality0
Sequence length25-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG12052.3102839E-517753.324265-269
CGGGTTT26453.09201E-514558.396270-273
TAACCGG7553.8545055E-514167.387265-269
GCCGGCC20206.4921034E-510590.473265-269
CCCCGGT14901.8641773E-46460.8994260-264
CCCGGTA9652.5926705E-45542.1646260-264
AACCGGG13402.5789687E-45526.2573265-269
ACGATCA20703.5978225E-44650.599260-264
ACCGGGT13454.2210185E-44337.835265-269
GTGCAAG90053.5836722E-44276.176270-273
TTACAAC102304.624945E-43764.1213270-273
CCGGTAA10305.9773715E-43665.235260-264
GTAAACC58855.509788E-43635.133265-269
CCGGCCA36008.762161E-42971.2163265-269
TACTCAA147309.5882744E-42614.186270-273
CCTAAAC84150.00112648032542.217265-269
CTTGCCG15000.00126760432516.7947260-264
GGGTTTA86550.00119164522471.7224270-273
TTGCCGG12600.00137002732425.4824260-264
GGCTTGT89000.00126005732403.6804265-269
Produced by FastQC (version 0.11.5)