FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25115698
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG1500.00954361210.3833739
CGTTGGA59550.010.2061441
AACGGGA55400.09.1554678
AAACGGG68600.09.1449317
ACGGGAT43050.09.0431629
GAAACGG73100.08.7341016
GGAAACG73650.08.5178515
AACGGGT14750.07.8427218
CGGGATT41500.07.77276210
ACCGCGC20600.07.020236531
GCGTGAG64850.06.92948822
CACCGCG22200.06.91523530
CCGAGTA41150.06.8141262
GCCCGAA5951.3871413E-66.7349811
ACAGGCG93600.06.72627618
GATCGCG9551.7546281E-66.63945245
CGTGAGC67800.06.562621623
CGCGCCC16450.06.49198533
CCCAAGT111050.06.41523031
GATTTCG29150.06.41053725
Produced by FastQC (version 0.11.5)