FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23957543
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN492780.20568887218526544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA50500.08.0689991
GGCGCGA16250.07.5364931
GCGCGAT16250.07.39621452
CGCGATC16250.07.12228063
ACGGGAT36400.07.08983049
AAACGGG59400.07.0412647
AACGGGA46250.06.9267588
AACGCGA3250.0069424236.8513571
CCTTCGT27100.06.81975461
CGCGCCC18750.06.6474623
ACCGCAC22500.06.63059141
ACCGCGC22250.06.60501671
GATCGCG9652.2039148E-66.5479345
GAAACGG62550.06.5443856
GCGTGAG65750.06.39517722
CGGGATA8356.6229404E-96.394461610
CCCAAAG256450.06.347091
GCGATCT32950.06.2819554
GGAAACG63350.06.1807955
ACAGGCG96900.06.15318918
Produced by FastQC (version 0.11.5)