FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20299906
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN948590.4672878780818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA44250.010.4159641
AAACGGG48850.08.744637
ACGGGAT31800.08.6755959
AACGGGA41150.08.59670458
GAAACGG51750.08.4265626
GGAAACG54200.07.8404115
CGGGATT31450.07.569511410
GCGTGAG63650.07.5504922
CGTGAGC62550.07.398908623
ACAGGCG92950.07.133360418
CACCGCG22100.07.04807830
ACCGCGC20600.06.80514231
CCGAGTA43600.06.73863172
CCCAAAG233300.06.63303761
AACGGGT11100.06.614488
CGGGATA8804.456524E-106.57348110
GAGCCAC167450.06.55101326
CGAGTAG44200.06.54644253
CGCGCCC15250.06.420140333
TATGCCG6000.00469037846.39971545
Produced by FastQC (version 0.11.5)