FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22336693
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2049830.9176962767048819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG1603.4556797E-715.2636036
CGACGCT2251.359522E-511.00205442
ACGGCGA2454.040462E-59.9680467
TATGCCG13654.1836756E-116.94325645
CGGCGAC3856.790279E-46.91990338
CACGACG5401.546105E-56.62196640
ACGACGC5401.0744183E-46.217736741
GATCGGA6050.00328972915.14501241
TACACGA9251.0799571E-55.06695538
Produced by FastQC (version 0.11.5)