FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9814008
Sequences flagged as poor quality0
Sequence length25-292
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG45750.031396.549270-274
TTAGGGG53350.026923.936280
GGGGGGG40101.2769173E-517910.125270-274
GTTACGT11903.1485568E-515088.151260-264
GGGAATT106900.013436.781275-279
GGGGGGA63459.590598E-57546.057270-274
AACCAAG106208.9561836E-56762.6743265-269
GAATTAG84201.6888941E-45686.4297275-279
GGAATTA127701.2949583E-45624.0874275-279
TTACGTT21603.3710923E-44749.9736260-264
GCGGCAC12953.675066E-44621.596255-259
ATTAGGG65603.4169765E-44379.2437275-279
ACCAAGG91203.9625805E-43937.4783265-269
GTTTTAA187152.7813375E-43837.542265-269
AATTAGG93804.1917278E-43828.3372275-279
GGGGAAT82305.378038E-43490.6245270-274
GGGGGAA108405.598093E-43312.7122270-274
TAGCGAG9508.082435E-43149.9824250-254
GGTTCCG10008.9555106E-42992.4834250-254
CCAAGGG96707.4245204E-42970.821265-269
Produced by FastQC (version 0.11.5)