FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20197579
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGA2607.508663E-59.4200131
CGTTGGA45800.08.94508651
AAACGGG50350.08.4835077
ACGGGAT30750.08.3924079
AACGGGA40850.08.1690758
GAAACGG52650.08.02837856
CGGGATT28550.08.02610510
ACCGCGC17100.07.806875731
GCGTGAG53200.07.611062522
CGTGAGC55050.07.55756123
CACCGCG19050.07.47489330
GGAAACG53950.07.42255975
AACGGGT12850.07.27143058
GATCGCG7804.485508E-67.198266545
CCCGAAA8402.0008883E-117.1567641
CGCGCCC12450.06.96975333
ACAGGCG80850.06.8518218
CCGCGCC18550.06.836820632
CCGAGTA34850.06.7693652
ATAGGCG10650.06.68477818
Produced by FastQC (version 0.11.5)