FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16885527
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN735778943.57452983256015No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTATGCCG297110.17595542028389163TruSeq Adapter, Index 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13650.040.4865761
TATGCCG90100.039.54446445
CGGAAGA49050.033.2101171
ATCGGAA13550.033.001782
TCGGAAG12150.029.2946223
ACACGTC75600.025.62831510
GCACACG73850.025.1212648
GTATGCC101250.024.67249544
CACGTCT80600.024.42536211
CACACGT78700.024.1384359
CGTCTGA82300.024.08262613
ACGTCTG83050.023.7312212
CGTATGC97950.023.6357243
CTCGTAT97050.023.04019441
TCTCGTA93200.023.0190640
TCGTATG99750.022.7078642
ATCTCGT95600.022.46457339
CATCTCG96350.022.26602438
AGAGCAC83950.021.8869555
GAAGAGC81250.021.7938463
Produced by FastQC (version 0.11.5)