FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83103289
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN994220.119636660830596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATC121100.016.558520
CGGATCA124200.016.32470321
GATCGAG83050.016.21191639
GACCATC214050.016.06085645
AGGCCGA249050.016.045558
GGCGGAT129650.016.03349119
AGATCGA87200.015.71896538
CGAGGCG86550.015.1335312
ATCGAGA91800.014.86326340
GGCCGAG263000.014.7293799
CGAGGTC118450.014.51460528
GCCGAGG262100.014.31322410
ACGAGGT122650.014.18049427
GAGGCCG289100.013.5766157
TCACGAG132200.013.43308225
ATCACGA138700.013.17279224
GAGTTCG157300.013.072597539
AGTTCGA163250.012.93925940
CACGAGG135850.012.93332626
GGATCAC462200.012.91921522
Produced by FastQC (version 0.11.5)