FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19181147
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN373771519.48639984876817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8700.027.6627391
ATCGGAA10450.023.2037982
TCGGAAG10850.018.8617363
TATGCCG36400.015.90547145
CGGAAGA19150.013.4983251
CGTTGGA33950.011.814721
ACACGTC27250.010.53000310
CGTCTGA28950.010.52647313
ACGGGAT25550.010.185929
AACGGGA35600.09.9345818
CGTATGC38200.09.650681543
AAACGGG42250.09.4765677
CGGGATT23850.09.41972510
CTCGTAT38600.09.17616341
GCACACG31050.09.1696098
TCTCGTA37800.09.126080540
ACGTCTG33950.08.84514312
TCGTATG41400.08.76138742
GGAAACG46100.08.7333915
CACACGT34750.08.6414029
Produced by FastQC (version 0.11.5)