FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18683042
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN754122840.36402637215075No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATCTCGTATGCCG232240.12430523894342259TruSeq Adapter, Index 16 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14950.038.7559281
TATGCCG73850.033.3222545
ATCGGAA15500.033.0116652
CGGAAGA41550.029.2301771
TCGGAAG14900.028.0672633
ACACGTC63200.021.96292110
CGTCTGA72000.020.57625413
GTATGCC85850.020.27512244
CACGTCT70650.020.08810211
CGTATGC80000.020.0809243
ACGTCTG73200.019.96543712
GCACACG66600.019.8396878
TGCCGTC32400.019.44982145
TCGTATG81350.019.3485642
CACACGT70050.019.275399
CTCGTAT80600.019.26301841
TCTCGTA76650.019.13307640
ATCTCGT78050.018.70325339
CATCTCG78850.018.45688238
AGAGCAC76050.017.9887285
Produced by FastQC (version 0.11.5)