FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41025934
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN574560.14004799988222083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA81650.010.0651941
TCGACGG2100.00107944339.53351726
AAACGGG103350.08.351057
AACGGGA85100.08.3121378
ACGGGAT62200.08.153779
GAAACGG108400.07.9209716
CGGGATT60600.07.634952510
GGAAACG110450.07.4920985
CGGGAAT43350.05.79835710
AACGGGT20650.05.7091438
TCGTTGG64750.05.53095539
GATTTCG43600.05.3570915
ACCGCGC31750.05.32479631
CCCTAAA116800.05.23398071
CCCCGAT15003.6379788E-115.22503331
CCCGAAT30450.05.22135641
GTTGGAA197350.05.21877432
CGAAACG7759.951026E-55.16650725
CCCATAA112600.05.1309011
TTCGTTG73450.05.1181158
Produced by FastQC (version 0.11.5)