FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25720843
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13179365.1240000181953596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21500.026.3005745
TAGCGAC18900.018.59879532
GCGACAT21100.015.92153334
CATAGCG22100.015.80482830
CGACATC21000.015.78611135
CGTATGC23950.015.58673843
CGGAAGA22850.015.4090761
ATAGCGA23150.015.28027531
CTCGTAT23250.015.133038541
ACACGTC24100.014.5842210
TCTCGTA23200.014.4821140
AGCGACA25250.014.09769433
TCGTATG25750.013.9948242
CGTCTGA26700.013.83068113
GATCGGA5950.013.1086141
ATCTCGT27750.012.02703939
ACGTCTG30750.011.71970512
CATCTCG29050.011.718638
CGTTGGA48600.011.4632941
CACGTCT32850.010.83517211
Produced by FastQC (version 0.11.5)