FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3747925
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA450.00395317231.6648666
ACGCGAG450.004306823531.11533557
CGCGAGT750.001818713823.36648258
CCCGTAT3400.022.3906821
CGCCCTA1055.109338E-420.20326463
CACGCTA1108.106488E-418.8644664
CGATTAT5400.018.84746659
CGCCTAG1158.600245E-418.6992367
ACGACTG7550.018.65206370
CCCCGCC8900.018.2739071
CGTATAG3002.0008883E-1117.92009767
AGATCGC5600.017.91216324
CCGACTA1402.0454053E-417.2924252
ACTATCG1854.6916593E-617.05121658
CTATCGG1653.320627E-517.01589459
CCGGTAT2853.1468517E-1016.9983481
TCCGCTA6350.016.88394429
AAGATCG6000.016.7180223
AGTGTAC5850.016.55453711
GATCGCC6100.016.4439525
Produced by FastQC (version 0.11.5)