FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076152
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACT6450.025.3337881
CCCTAAC4650.023.925391
CCCGTAT1553.7718564E-822.4300541
CGGGTTA2108.913048E-1121.56284355
CGCTAGT1206.024033E-520.2805061
GTCCGGT2102.1391315E-919.8666171
ACGACTG6300.018.32674870
GTCCGAT3103.6379788E-1217.9440421
CGAAACA3205.456968E-1217.41983257
ATGAACG1602.7718283E-517.3776433
CCTAACA6000.017.3773572
CCTACTG9350.017.0985772
CGATTAT3900.016.98285559
ACCCATC9200.016.91499970
GGCTCGC8750.016.73667560
CCCCTAT3359.094947E-1216.6049351
GATAACG3359.094947E-1216.59926627
TACACCC5450.016.5802319
ATCGTCG1704.6602712E-516.35595913
CCCGAAT7500.016.2244051
Produced by FastQC (version 0.11.5)