FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30088381
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC449350.14934336280838775No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC334580.11119907049834286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36850.029.8470486
CGGTATA55450.026.81092820
TCGACGA43100.025.9918967
AGACCGC190950.023.0215234
CAGACCG194950.022.6200563
CGACGAG48750.022.1283728
GTTCGAC49550.021.9177325
GACCGCG205500.021.1054845
ACCGCGT223750.019.5696226
TACGGAG227800.019.46857834
CCTACGC20550.018.99602965
ATACGGA235700.018.97499833
AATACGG235550.018.9259232
CCGCGTT237750.018.483977
TATAGAG87650.018.30252523
CTACGCC21400.018.30012966
GCGTTCT242300.018.2938699
GTATAGA88450.018.21516222
CTCAGAC283650.018.0192361
CGCGTTC245250.017.918688
Produced by FastQC (version 0.11.5)