FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32863605
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC839650.2554954028932614No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC768210.23375706956068878No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC572140.17409532520853996No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA488210.14855643499853408No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA355990.10832347820636233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33350.032.84256
ACCGCGT424500.026.87176
AGACCGC425800.026.8382974
CCTACGC40250.026.7607265
GACCGCG429950.026.5148745
CAGACCG432950.026.45923
CGCGTTC441450.025.949378
CCGCGTT442650.025.9338977
TCGACGA43950.025.3077187
CTACGCC42350.025.2441866
GCGTTCT478800.024.20029
TCAGACC490500.023.5470282
AATACGG477650.023.4575232
CGACGAG48000.023.388878
ATACGGA481150.023.27431933
TACGGAG480950.023.17112734
CTCAGAC540000.022.6954771
GAACGAT494000.022.5457245
CGTTCTC534750.021.90765610
ACGGAGA512800.021.80185535
Produced by FastQC (version 0.11.5)