FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27623845
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC437890.15851884486030096No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC331940.12016430008204868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG42700.035.920046
TCGACGA47300.032.489677
CGGTATA67150.030.28867320
CGACGAG51350.028.985618
CAGACCG193050.024.10293
AGACCGC192100.024.096444
GACCGCG199850.023.1276825
CGAGGAT76700.022.69163511
ACCGCGT207400.022.4190926
GTATAGA102500.022.13362122
GTTCGAC70100.021.5842155
CCGCGTT217550.021.4775687
AATACGG224050.021.04858632
ATACGGA224300.021.02901533
TACGGAG223650.020.92430934
CGCGTTC225900.020.7447978
CAGTTCG76750.020.2987673
GCGTTCT234750.020.095169
GAACGAT235700.019.85735545
ATAGAGT113100.019.66233324
Produced by FastQC (version 0.11.5)