FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23205849
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG126700.028.94501750
CGTCGAA91550.028.43877864
TCGTCGA95900.027.23761463
GTCGAAC98750.026.57396765
CTCGTCG98500.025.92709562
TCCTCGT138850.023.20720960
TCGAACT114600.023.107466
ATCCTCG129400.022.88473959
CGAACTG113600.022.87726467
TCTATAC177850.022.83621248
CCGTCAT157000.021.84600354
CTATACC172850.021.30246749
TCGTTGA33650.021.00816763
TATATAC88200.020.9244617
CCTCGTC155450.020.6782361
TACCGTC191550.020.19581452
ACTCTAT202900.020.14584246
ATACCGT196200.020.0896951
CTCTATA206100.019.87949647
CGTCATC174050.019.18653355
Produced by FastQC (version 0.11.5)