FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24889381
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC433910.17433539227030195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA23600.019.96173564
CTCGATT78850.017.36289249
TCACCCG83900.017.2376373
ACAGTCC153250.017.19631270
ATAGCTC82250.016.76457639
ACGGACA86050.016.40994818
TCTCGAT84900.016.3245148
CGGACAG87750.016.01302319
ACACGGA87100.015.85415416
CGGACAC95950.015.76172713
TTCTCGA87550.015.74535347
ACCCGGC89100.015.7272565
TCGTCGA31050.015.719316563
TAGCATA67250.015.59034259
GTTGTCG26700.015.55470626
CCGGACA98550.015.45111712
ATTTCTA261450.015.4038582
CACGGAC91350.015.268417
TTTCTCG90200.015.23772346
TCTATAG262750.015.2358365
Produced by FastQC (version 0.11.5)