FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637635
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGTTAACAATTTGCAGGCAAATTAATGGCCTTTTCTAACATAGTTC27010.10240234149152555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA350.001303704839.6982773
CTCGCTA500.007380264327.86803857
CGACCGT500.007483558327.7887921
CCCGTAT1401.2925739E-824.8114221
CGGTCTA1052.1650594E-523.1573284
GTCGCAT800.002787922721.70999516
CCCTACT3550.021.5265271
CCCCTAT1356.437802E-620.5842911
TGGCCTA6550.019.6879259
CTCGTTT5600.019.2288531
ACCGCAT1301.1023176E-418.74628455
CGCGGTT950.007534998518.28211
GCCTACG2306.688424E-918.12312510
CCCGAAT2902.0008883E-1117.9668921
CGGATAC4650.017.9282533
CCGACGT1954.28141E-717.8133281
CGCCTCA6750.017.4966474
CCCCGCC6200.017.3679961
CCTACTC7300.017.2044462
GCCTACT7100.017.19279961
Produced by FastQC (version 0.11.5)