FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25649024
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC842450.32845304367136935No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC558450.21772758292869154No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC406560.15850895535050377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG82900.039.489836
TCGACGA91400.036.18377
CGACGAG92900.034.7827268
GTTCGAC95000.034.6051865
CGGTATA124950.033.2282220
AGTTCGA110600.030.2229124
CAGTTCG113500.029.6634453
ACGAGGA136100.028.3783910
TATAGAG157950.028.20718423
ATAGAGT158450.028.09646824
CGAGGAT129450.027.91817911
GACGAGG142750.026.8880169
ATGACGG145850.026.52802816
GTATAGA167150.026.48960522
AGACCGC379900.025.8151824
GACCGCG375050.025.7261735
ACCGCGT377700.025.7193076
CAGACCG391600.025.1936453
TAGAGTT176650.025.1041225
CCGCGTT394250.024.675847
Produced by FastQC (version 0.11.5)