FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25649024
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC358060.13959985378001127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335480.1307963998942026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA78750.029.32534464
TATACCG118000.028.09649750
GTCGAAC89500.026.11586865
TCGTCGA89350.025.82135663
CTCTATA148350.024.14026647
CTCGTCG95300.023.92579362
CGAACTG97150.023.63329567
ACTCTAT153050.023.3825546
TCTATAC156500.022.8788148
TCGAACT103350.022.4686366
CCGTCAT142700.021.81712354
AACTCTA168200.021.57083745
TCGTTGA34350.021.28632563
TATATAC79650.021.2811477
CTATACC158250.020.86586249
ATCCTCG124950.020.8078359
TCCTCGT137100.019.97693460
GTTGTCG38050.019.83353426
CCATATA84450.019.5804424
TACCGTC184250.019.38933252
Produced by FastQC (version 0.11.5)