FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259026
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC2650.020.922059
CATACGT1055.8526784E-419.8000119
CCGCGTT2801.2732926E-1118.5639348
GTCGACT2351.6748163E-716.2675821
TCCGCGT3855.456968E-1215.3009467
TTCCGCG3503.5470293E-1014.8509216
GTTGCCG6101.1168595E-913.75929970
AATTCGC2557.176037E-613.58824316
GCGTTCC4456.91216E-1113.23789810
TAGCATA3153.2769276E-713.2008179
CGATAGT3050.001952482312.84201270
TAATTCG2701.2936018E-512.8333415
GCGCGGT2255.170119E-412.32076411
TTAATTC15950.012.09174368
CTTAATT16600.011.91401867
CGTAGCG2357.0269906E-411.87923361
GTCCGTA2357.141793E-411.85633258
TAATTCT18150.011.45402569
GCCCGGT3950.00104274611.33255370
ATCGGTT11450.011.23976570
Produced by FastQC (version 0.11.5)