FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16912946
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258070.15258725475739118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255370.15099084452820935No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201150.1189325620740467No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185470.10966155748383516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG5550.015.4056353
CCCCCCC217150.015.08976651
GTCCGAT8200.013.5038171
CGCCTAT6250.013.3781491
CGAATCG4300.013.07926918
CCCGTAT9850.013.0771461
GTATCGA5600.012.8574864
ACGTTCG5450.012.7962746
CCGTATC11050.010.7923982
GTCCGGT13300.010.7044221
GTCGGTT21600.010.6713851
CCCCCCT110200.010.4131634
GTCCGTT10100.010.2922611
CCGATTG9100.010.1375133
GCACGAC16250.09.82937424
GTCGGAT11300.09.7992311
GCGAAAA4853.6379788E-119.78481
CCGGTAT13900.09.754631
CCCCGAT30500.09.5579391
CGTGCGA7900.09.39837438
Produced by FastQC (version 0.11.5)