FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505180
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220060.13332784010837812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT9400.015.1449781
CGCCTAT7250.013.4025171
CCCCCCC189550.013.1256091
CGTATCG6100.012.9099043
CCCGAAT11500.012.3793741
GTCCGAT8500.012.229111
GTCGGTT20150.012.2238331
GTCCGGT12250.011.068051
CCGTATC11000.010.8413922
TAGTGAC56650.010.20669337
GTCGGAT8700.010.1298091
GATCTAG15650.010.0629435
GTCGGAA15650.09.9630131
CCCCCCT87800.09.9174774
CGATATA4904.9112714E-119.6429074
GCACGAC15550.09.54864424
CGATTGG7800.09.51927954
CCCCGAT29900.09.4470181
TCCGATA6500.09.346572
GTCGTTA8150.09.1498151
Produced by FastQC (version 0.11.5)