FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19499669
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA831220.4262739023929073No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435470.2233217394613211No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314820.16144889433764234No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297690.15266412983728084No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202910.1040581765772537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG12800.017.923146
CCCCCCC303450.015.6816451
GTATTGC53150.014.60004511
CGTTCGA16600.014.4977167
ACCCTAC53850.014.03419426
GCAACCC55800.013.86615123
CGCCTAT8150.012.8693481
GCGATCG18800.012.68342441
CGTATCG8750.012.5962713
AGGAATC70850.012.5748512
CGTGCGA17900.012.31576938
GGCAACC65150.012.014276522
CCCGTAT10650.011.9893261
AACCCTA64850.011.89643825
CCTACAA66050.011.8169428
TATTGCA66100.011.80772912
GGAATCT97350.011.6452433
TGTATTG66800.011.5493110
GTCCGAT11500.011.4996991
CGATCGG20750.011.49080942
Produced by FastQC (version 0.11.5)