FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20112067
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA355140.17658055733406217No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295380.14686705250136647No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243690.12116606413453178No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231730.1152193854565023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC257950.015.231591
ACGTTCG9700.014.1445656
GTATTGC45300.012.31378211
GCAACCC48500.011.6403123
ACCCTAC46900.011.55789926
CGCCTAT8050.011.3307751
CGTTCGA12100.011.1531457
CCCGTAT11200.010.7907931
CCCCCCT132700.010.780673
CGTATCG6300.010.7112523
CGTGCGA12500.010.07754738
GCGATCG13200.010.05439741
GTCGGTT20950.09.90496351
GATCTAG17850.09.8287585
CCTACAA57300.09.8137328
GGAATCT94650.09.7912053
GTCCGAT11000.09.7431791
GGCAACC59600.09.62340222
AGGAATC69100.09.5400022
GTCCCGT19200.09.3825611
Produced by FastQC (version 0.11.5)