FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16726673
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471840.2820883746576501No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309850.18524305461103951No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234130.13997404026491103No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218810.13081501623185915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC236650.015.6769981
ACGTTCG6450.015.3431396
GTATTGC45200.012.98817111
CGCCTAT6500.012.629061
CGATTGG7750.012.4803554
ACCCTAC47800.012.18707926
GCAACCC49250.012.10221223
CGTATCG5600.012.0501783
GTCCGAT11250.011.9586291
TCGCGAT2201.0645081E-511.24733639
CGTTCGA10000.011.2458247
GGAATCT88250.011.1385053
GTCCCGT14150.011.1192251
CCTACAA54950.011.01139128
AGGAATC61750.011.0033182
TGTATTG54700.010.93805810
CGTGCGA9500.010.89215738
TATTGCA55900.010.82396512
TTCGCGA1903.8812924E-410.65537238
GCGATCG9400.010.52945241
Produced by FastQC (version 0.11.5)