FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068860
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354300.22048857230693403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260800.16230149494114704No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210650.13109206253586128No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208050.12947402615991427No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCAC163400.1016873630114395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG12600.018.747056
CGTTCGA15050.016.4425957
GTCCGAT8600.016.2919861
CCCCCCC213900.015.6784791
CGTATCG7500.014.40082553
CCCGTAT9650.013.3483731
GCACGAC15150.012.47347724
GCGATCG19050.012.40112941
CGTGCGA19250.012.27220838
CGCCTAT7200.012.2408881
CGATTGG7600.012.1387784
GTCCGTT7850.011.803071
GTCGGTT20500.011.7953461
CCCCCCT109600.011.0658723
CCGTATC13050.011.0355272
ACGACTT19750.010.9846171
TAGTGAC71100.010.79058337
TGTTACG8550.010.5269193
GTCCGGT9700.010.4838691
AGACGTT23700.010.3486294
Produced by FastQC (version 0.11.5)