FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16864883
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281310.1668022244803003No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276300.16383155459779947No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242070.14353494180777893No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237090.14058206036768828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT952.5486504E-416.802011
CCCCCCC254450.015.5483331
GTCCGAT7850.012.2001851
CGCCTAT8950.011.9746181
GTCGGAT11950.011.258251
CCCGTAT8600.011.1362161
CCCCCCT129300.010.8726213
CGTATCG5650.010.7493
ACGTTCG5500.010.6323996
GTACGAT12000.09.74754936
GTCGAAA24300.09.4776761
CCGATTG8550.09.2077753
GTCCGGT11250.09.1210921
TAGTGAC55750.08.99781837
CCCCCTT159950.08.9860614
GTCCGTT9700.08.9330271
TTACCGG4854.100002E-98.8111738
GCGTATA2300.00223060168.8036492
GTCGGTT20750.08.7914131
CCCGAAT12850.08.6952041
Produced by FastQC (version 0.11.5)