FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13854910
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235540.17000471313057972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220780.15935145013572807No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187010.13497741955739878No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180260.13010550050487518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC196800.015.8260481
ACGTTCG4600.015.1577486
CGTATCG5800.014.7373493
GTCCGAT6250.014.5924031
CGATTGG5950.011.3414364
CGTGCGA5800.011.24745138
CGAATCG3602.237357E-1011.24586918
GTCGGTT17850.011.2406461
CCGTATC10450.011.1951712
GTATCGA6100.011.0625494
GCGATCG6350.010.98172441
CCGATCG3103.634341E-810.8841213
CCCGTAT9950.010.5409951
CGTAACG1500.00880312810.49712853
CCCCCCT103050.010.3683433
CGATCTA2402.7883212E-510.3096814
GTCGGAT8150.010.0714441
CGTTCGA6600.09.8829127
GCACGAC13200.09.88287624
AGCCCGC29300.09.82620314
Produced by FastQC (version 0.11.5)