FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005193214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005193214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19621896
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA603580.3076053404828973No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438840.22364811229251239No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341640.17411161490204616No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321820.16401065422016303No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206230.1051019738357598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC342950.016.5344181
GTCCGAT10250.013.7915091
GTCGGTT26350.013.4985751
CGTATCG7200.012.1840483
CGCCTAT9450.011.822471
CCCGTAT10600.011.1851411
CCCCCCT176300.011.1000884
ACGTTCG4700.011.0067346
TAGTGAC80250.010.95990237
GTCCGTT11600.010.41745951
TCCGATT22950.09.9013012
CGAACGC3653.7473E-89.859591522
CGATTGG8250.09.81544
CTCCGAT20950.09.7949551
GATTCAC97050.09.75804145
GCAACCC27200.09.59223523
GTCGAAA23300.09.5899011
CTCCGGT34650.09.5413261
CCCGGTT16850.09.4720091
CCCGAAT13500.09.4579761
Produced by FastQC (version 0.11.5)