FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005198373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005198373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39050935
Sequences flagged as poor quality0
Sequence length26
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGT1869550.019.672917
ACGCTCT1505400.019.6438067
GAGCAGT1767850.019.6243389
TCATGTC1850500.019.6085648
CGCTCTT1510300.019.5735288
TGAGCAG1772750.019.5677388
GGCTTCA1387550.019.5632518
CATGTCT1846200.019.559559
TGGCTTC1400550.019.545097
ATGTCTT1848350.019.54181710
GTAATCT1320900.019.5330249
AAGAGAC1150250.019.52728
ATCTCGC352400.019.511198
CAAGAGA1165750.019.4870837
AGCAGTT1781350.019.4819410
TAATCTT1528850.019.47167610
GCTTCAT1397850.019.4678429
CGGTAAT1339350.019.4578937
GCAGTTT1963900.019.45369511
ATGAGCA1796700.019.45327
Produced by FastQC (version 0.11.5)