FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005198385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005198385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28040148
Sequences flagged as poor quality0
Sequence length35-76
%GC20

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAGT474400.068.072379
ACTAGCG256650.067.525927
GATCGAA478450.067.416847
CGAAGTT480300.067.2215110
GATTCGC273500.067.1568
GTAATCT655800.067.082229
ATCGAAG482000.067.056978
TAGTTCC512800.066.909988
AGACAGC350550.066.796268
GACAGCT345350.066.7819
GCGGTAT394750.066.754968
AGTACGT187850.066.721117
TGATTCG279050.066.6720357
CGCTATA196350.066.588757
CTAGTTC522150.066.501767
CCTGCTA280850.066.393897
CTAGCGA260500.066.299158
CCTGACA345950.066.292737
GTACGTG187450.066.266548
ATTCGCT275650.066.26499
Produced by FastQC (version 0.11.5)