FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005198399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005198399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38149582
Sequences flagged as poor quality0
Sequence length26
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAA611480.16028484925470482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACT530000.019.6196927
GGAACGT938400.019.6142929
AGGAACG940450.019.6087518
GAACGTT941900.019.59345210
GTAGAAG625800.019.5739368
TAGGAAC946950.019.5628037
CGTAGAA626500.019.532867
GTGACTA527750.019.5196028
GACGATT358650.019.48319810
TAGAAGT629400.019.4826329
AGTTTCG422100.019.4801127
AATCGAT411200.019.4737059
ACACTTT805550.019.45795611
ATCGATT410900.019.45144510
ATTGGAC484450.019.4457427
TGACTAT529000.019.4432329
GAACACT540550.019.438469
CAAAGTG563850.019.4190067
AGTGGTT551150.019.41660710
AAGTGGT554350.019.4055129
Produced by FastQC (version 0.11.5)