FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005198634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005198634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24255676
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG103100.093.481091
CGTGTAC73500.093.3503341
CGTGTTG127700.080.393891
CGTGTTC105250.079.699841
CGAGTAC56350.076.117031
CGGTATG28600.075.624641
CGAGTTG86550.071.676741
CGAGTAT31950.071.58321
ATACTAG216400.071.313921
CGGTACA37600.070.9320761
CGGTACG5500.070.412671
ATACTTG327050.069.5285341
CGTAGGG38700.069.390472
CCGTACA41850.069.315871
ACGTAGG58750.068.281351
CCGTTGT46200.068.0086441
TCGTAGG39650.067.264721
CGGTACT31200.066.2780151
CGTTGTC47550.066.014292
CCGTTGC56500.065.568791
Produced by FastQC (version 0.11.5)