FastQCFastQC Report
Sat 28 May 2022
EGAF00005199471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33260043
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG1012100.30429906539808144No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC993040.2985684654707151TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA369880.11120851527461947No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG343240.10319890446323235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)