FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005199531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53572394
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT4744990.8857155048923145No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG3598310.6716724289006013No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1220370.22779829477099717No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA1135570.2119692466982155No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA968110.1807106100205266No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC935380.17460112012168058No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA912120.17025933169983035No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG896550.16735298407608964No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC895370.16713272137885044No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG838120.15644624729669537No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCG821640.1533700360674567No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG817990.1526887150124372No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC802820.1498570327097945No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT780400.14567204146225013No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC762180.142271036086235No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA728380.13596181645345173No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA727460.1357900862149263No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG725170.13536262725164008No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC712080.13291920461870715No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC701470.130938706976582No Hit
CGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAA691380.1290552742518843No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG657210.12267698919708535No Hit
GGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA623150.11631923710558836No Hit
TGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTA596610.1113651930507343No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG586870.10954709248199734No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT581380.10852231094992694No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG580310.10832258121598973No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA571460.10667061098669588No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG564560.1053826341977549No Hit
GGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAAC561210.10475731213355893No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA554290.10346560207856308No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT550760.102806680619873No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG538230.1004677894364773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)