FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005199569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109457679
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT9001580.8223799446724975No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG5188500.4740188214661486No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC4579280.41836078033410523Illumina Single End PCR Primer 1 (96% over 33bp)
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC2626380.23994479181309883No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1536090.14033643085013708No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG1188280.10856067942021683No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC1158050.10579888141059524No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC1147840.10486610080595625No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC1130010.10323716072949071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)