FastQCFastQC Report
Wed 13 Jul 2022
EGAF00005199653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517633771
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC66818781.2908504766007627Illumina Single End PCR Primer 1 (96% over 31bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT19966300.3857225150018274No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG9366160.18094182653318422No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG8728590.16862481717793484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)