FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005199782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71687515
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTCTCCAAACTGG2536530.35383148655662006No Hit
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC2429090.33884421855046865No Hit
CCCGAGTTTAATTCCCCGAACCATTCTCCAGGGCAAGTTTGCACAGTAAT2233110.3115061248810201No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC1984980.27689340326554773No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT1891020.26378651847535795No Hit
CCCCACTCTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGGGCTTTC1881130.2624069198102348No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC1766550.24642366247456057No Hit
GGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGA1730030.2413293304977861No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT1705790.2379479885723476No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA1698180.23688643692001318No Hit
CTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGA1608970.2244421500731334No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT1589860.22177641392646963No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC1490110.20786185711696104No Hit
GTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGGTGATGGTG1432000.19975584311996306No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA1402650.19566168530182698No Hit
GTCATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGG1368420.19088679528087982No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC1300000.18134259501113967No Hit
CCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGACTGGC1288340.17971609142819361No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG1284380.1791636939849289No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1284340.1791581142127747No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC1210490.1688564598731034No Hit
GTCGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGT1196320.166879825587482No Hit
CACTATTCCAACTAATACCTGAGATCCACTCCAGGCCCTTCCCTGGAGCT1139580.15896491878676502No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG1137050.15861199819801258No Hit
CCCCAGACTCCTCCAGTTGCCCTTCACACTGGACACCTTTTAAAATAGCC1118240.15598811034250526No Hit
CGTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGC1061160.14802577547847764No Hit
CGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCC1059880.14784722276954362No Hit
GTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATG1058140.14760450268083639No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG1039570.1450140934582542No Hit
GCTGAAGATACGCTGACCATTGTCCCTTGGCCCCAGAGAGCGAAGGCGAC1019700.14224234164066085No Hit
CCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAG1002480.13984024972828255No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG995060.1388052019936805No Hit
GTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGCGGCCGAATAACCA983010.13712429563223108No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG973510.13579909974561122No Hit
CTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGACTGGCC959260.1338113059156814No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT938730.13094748785754395No Hit
CCCACTATTCCAACTAATACCTGAGATCCACTCCAGGCCCTTCCCTGGAG932210.13003798499641117No Hit
GTCCCACTATTCCAACTAATACCTGAGATCCACTCCAGGCCCTTCCCTGG927040.12931679944548224No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTT903920.12609169114036106No Hit
GCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTG902000.12582386207695997No Hit
CCACTATAGTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT886820.1237063385444453No Hit
CTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTT885250.12348733248739338No Hit
CTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCA872070.12164879756258813No Hit
CCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTG870640.12144932070807586No Hit
GCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGCAGATACAGGGAGCTCTT868230.121113139435786No Hit
GTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC865370.12071418572676149No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACC860990.1201032006758778No Hit
CTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCA860660.12005716755560573No Hit
CTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGC860070.11997486591633144No Hit
GTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCA859210.11985490081501639No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCT852540.1189244738083054No Hit
CACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTT848730.1183930005106189No Hit
CGGGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAG847950.1182841949536122No Hit
CCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACC843210.11762299195334083No Hit
CTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCG837350.11680555533275215No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA833420.1162573427186031No Hit
CTCCAGGGCAAGTTTGCACAGTAATACAAGGCCGTGTCCTCAGCTCGCAG832470.1161248231299411No Hit
GTCCTCAGCTCGCAGGCTGTTCATTTGCAGATACAGGGAGCTCTTGGCGT827980.11549849370563339No Hit
GGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCA826360.11527251293338873No Hit
CCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAG818450.1141691129898979No Hit
CCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTG816330.11387338506572589No Hit
GTCATCTCCTCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGG815090.11370041212894604No Hit
GTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGCGGCCGAATA812210.11329866853384443No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT811840.11324705564141817No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAG806680.1125272650335278No Hit
GTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTC796590.11111976750763367No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTCTCCAAACT794490.11082682946953874No Hit
CCACTCTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGGGCTTTCCC793420.11067757056441418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA790790.11031070054527627No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTG774240.1080020698164806No Hit
CCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGG765650.10680381374636853No Hit
GTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCT754470.10524426742927273No Hit
CACAGTAATACAAGGCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGCAGA752870.10502107654310516No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAA747020.10420503486555505No Hit
GGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCAT741000.1033652791563496No Hit
GGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCA740540.10330111177657643No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT735750.10263293406111232No Hit
CTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGA733580.10233023142174756No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT732200.10213772928242804No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA719620.10038289093993565No Hit
TGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCT719260.10033267299054793No Hit
GCACAGTAATACAAGGCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGCAG718500.10022665731961834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)