FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005199783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71687515
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC4097040.5715137426649536No Hit
CTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGG2689500.375169930217277No Hit
GGAGCCCCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCACT2481430.34614535041422484No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC1959040.2732749210235562No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT1906410.2659333358116821No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT1821270.254056790781491No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCA1745440.24347893772018742No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG1593130.22223256030007454No Hit
CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTG1591230.2219675211227506No Hit
CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCC1559460.21753578708928606No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATC1413690.1972017024163831No Hit
CGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA1400950.19542454498527395No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACG1355950.1891473013118114No Hit
CCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAG1318060.18386186213875597No Hit
GTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT1303310.1818043211568988No Hit
CATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGC1280060.1785610785922765No Hit
GTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAA1272760.177542770174137No Hit
GTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC1245430.17373039084978745No Hit
CCCCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCACTGAAC1218440.1699654395887485No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT1196290.16687564075836636No Hit
GGGACAATGGTCAGCGTATCTTCAGCCTCCACCAAGGGCCCATCGGTCTT1191770.16624512650494302No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGT1189720.16595916318204082No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT1188630.16580711439083917No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC1182620.1649687536246723No Hit
CACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACA1174800.16387790816852837No Hit
CGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCA1140940.15915463104000743No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG1132170.15793126599520155No Hit
GTCGTCGCCTTCGCTCTCTGGGGCCAAGGGACAATGGTCAGCGTATCTTC1128260.15738584326712957No Hit
CTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGACAT1126060.1570789557986492No Hit
CCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACA1093060.15247564377144332No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC1071470.14946396175121987No Hit
GGTTATTCGGCCGCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG1054910.14715393607938565No Hit
CCCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCACTGAACA1052680.14684286378178962No Hit
CCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCACTGAACAC1036500.1445858459454202No Hit
ATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCGACTTATT1004590.14013458270941598No Hit
CTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTT1003040.1399183665384412No Hit
CTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCA996160.1389586457279207No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG995640.13888610868991622No Hit
AGGAGCCCCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCAC987610.1377659694299628No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG984660.13735446123359138No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTCCAGTT984130.13728052925254836No Hit
CCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGT983500.1371926478411199No Hit
CGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGC968850.1351490562896482No Hit
AGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGC957090.13350860327631667No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC941280.1313031983323735No Hit
CCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGA940230.13115672931332603No Hit
TGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT937380.13075917054734007No Hit
CCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAGAGTGGGGTCCCAT913890.12748244934979264No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT913410.12741549208394237No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT907920.12664966835577993No Hit
GGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGA883160.12319578939233701No Hit
GCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC866450.1208648395749246No Hit
CTTGGTACAGCCTGGCGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGAT863640.12047286058109281No Hit
GCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA862430.12030407247342861No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG859610.1199106985365583No Hit
GGCCGATTCGCCGCATCCAGAGACAACGCCAAGAGCTCCCTGTATCTGCA836710.11671627897828513No Hit
GTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGA834670.11643171059842149No Hit
GCCCCAGCCCTGAGATTCCCAGGGGTTTCCATTCAGTGATCAGCACTGAA831180.11594487547796851No Hit
CACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAG830170.11580398623107524No Hit
CAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCC828420.11555987119932946No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTC819090.11425838934436491No Hit
CAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAA813550.11348559090100976No Hit
TGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA813380.11346187686935445No Hit
GCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAG812260.11330564324903715No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT808640.11280067386908307No Hit
GTGGGACCATAGTCTATGCGGACTCTGTGAAGGGCCGATTCGCCGCATCC801260.11177120590663521No Hit
CTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGA797700.11127460618491239No Hit
CCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCT783940.1093551645638714No Hit
CGGCCTTGTATTACTGTGCAAACTTGCCCTGGAGAATGGTTCGGGGAATT783890.10934818984867868No Hit
ATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCA781260.10898131982954075No Hit
CTCAGGTATTAGTTGGAATAGTGGGACCATAGTCTATGCGGACTCTGTGA777920.10851540885466598No Hit
CACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTG769070.10728088426555167No Hit
CCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGC761000.10615516523344405No Hit
CGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGG759040.1058817563978888No Hit
CCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAA755480.10538515667616599No Hit
CCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC753070.1050489754038761No Hit
CGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTG745310.10396649960596345No Hit
GTGAAGGGCAACTGGAGGAGTCTGGGGGAGGCTTGGTACAGCCTGGCGGG743380.10369727559952385No Hit
GGGCAACTGGAGGAGTCTGGGGGAGGCTTGGTACAGCCTGGCGGGTCCCT740310.10326902808668985No Hit
CCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGAT731550.10204705798492247No Hit
CGCCAAGAGCTCCCTGTATCTGCAAATGAACAGCCTGCGAGCTGAGGACA721970.10071070255399422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)