FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005199994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005199994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74919780
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT1693310.22601641382289164No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1330390.1775752678398148No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG1274800.17015533147587994No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG1236360.16502451021612716No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT1226320.16368441017846022No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1173610.1566488849807087No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1140330.1522068003937011No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG1120950.14962003358792567No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG972040.12974410763085528No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC967960.12919952514542887No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA935020.12480282243220683No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA927680.12382310786283675No Hit
GATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCCCGC889400.11871364277898305No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG887960.1185214371958914No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA879670.11741492033212057No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA871650.11634444201517945No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT870100.11613755406115714No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC864910.11544481310543091No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT860340.11483482733131357No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC852610.11380305708318951TruSeq Adapter, Index 5 (100% over 50bp)
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT841940.11237886710291996No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC829340.1106970682508678No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT814380.1087002658043043No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA812080.10839327077575509No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC804200.10734147911272564TruSeq Adapter, Index 5 (100% over 50bp)
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG797850.1064939058817311No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG788620.10526192148455321No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT753770.10061027942153593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)