FastQCFastQC Report
Sat 25 May 2024
EGAF00005200121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67717507
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1062030.15683241262853934No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1001300.14786427385757128No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT968610.14303686637489474Illumina Single End PCR Primer 1 (100% over 50bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT883980.13053935963708765No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC761650.11247460719426662No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC758200.1119651377597229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)