FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10788170
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232220.21525430170269844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT683350.074.258891
AGCAGTG764650.066.2085342
CAGTGGT992050.050.947594
GCAGTGG1023100.049.5686343
AGTGGTA1036650.048.770455
TGGTATC1078050.046.78767
GTGGTAT1080050.046.7153976
GGTATCA1104600.045.7711568
GTATCAA1123000.045.0405469
CAACGCA1107750.026.29670512-13
ATCAACG1106950.026.25862510-11
ACGCAGA1158000.025.18976214-15
AGAGTAC1144650.025.06607218-19
GCAGAGT1157900.025.01541516-17
TACATGG1129200.024.8551522-23
AGTACAT1143300.024.79293320-21
TCAACGC1100200.023.0497312-13
CATGGGG447650.022.99422324-25
CATGGGA467950.022.65008524-25
CGCAGAG1125900.022.60444516-17
Produced by FastQC (version 0.11.5)