FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10616549
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN252070.23743120292667608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT663250.074.12741
AGCAGTG746450.065.765982
CAGTGGT963650.050.7855724
GCAGTGG994700.049.366273
AGTGGTA1006050.048.6334575
TGGTATC1055500.046.241517
GTGGTAT1056700.046.1832246
GGTATCA1080400.045.309748
GTATCAA1100500.044.5399069
CAACGCA1083700.026.08506412-13
ACGACGC4400.026.078185
ATCAACG1084850.026.01084910-11
ACGCAGA1133800.024.93947214-15
AGAGTAC1121250.024.77052118-19
GCAGAGT1138200.024.63415116-17
TACATGG1106900.024.57029322-23
AGTACAT1119950.024.55649620-21
TCAACGC1075000.022.88067412-13
CATGGGA447050.022.80539924-25
CATGGGG442300.022.67582524-25
Produced by FastQC (version 0.11.5)